Main research interest

My main research interest is focused on searching of bioactive compounds maninly from algae and cyanobacteria. I design appropriate methods of their detection and isolation with use of HPLC-HRMS techniques. With high resolution mass spektrometry I am able to elucidate structures of majority of bioactive compounds, especailly peptides and lipopeptides. Nowadays I deal with metabolomics with use of GC-APCI-HRMS technique.

 

Scientific expertises

 

HPLC-HRMS/MS techniques for small molecules identification, compound purification, interpretation of mass spectra

 

Description of the academic carrier

• 2019–now     Junior scientist, Center Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic
• 2016–2018    Maternity leave
• 2013–2016    Junior scientist, Center Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic

Education

• 10’ 2013  Ph.D. in Agriculture Chemistry, University of South Bohemia, Ceske Budejovice, Czech Republic, Advisor: doc. Roman Kubec, Ph.D.
• 6’ 2009    MSc. In Consumer Chemistry, Brno University of Technology, Advisor: prof. Miloslav Pekař, CSc.

Most important research projects

• 2019 Project Strategy AV21, programme Diversity of Life and Health of Ecosystems, Health and Longevity of Bees – co-investigator
• 2016 Project Strategy AV21, programme Diversity of Life and Health of Ecosystems, Health of Bees – co-investigator
• 2012 Grant agency of University of South Bohemia – Undesirable sensory changes of onions and garlic during processing (GAJU 060/2012/Z) – principal investigator
• 2011 Grant agency of University of South Bohemia – Study of the formation of undesirable color in the garlic and onion during processing (GAJU 067/2011/Z) – principal investigator

The most important poster presentations (11 in total)

• Kust, A, Hrouzek, P, Urajová, P, Hájek, J, Saurav, K, Voráčová, K, Mareš, J, Jokela, J, Fewer, DP, Sivonen, K 2017. First record of a putative pederin-type gene cluster and its product in a free-living cyanobacterium Cuspidothrix issatschenkoi. International Summer School on Natural Products, Naples, Italy

• Kust A, Mareš J, Hrouzek P, Delawska K, Čapková K, Urajová P, Kozlíková –Zapomělová E, Řeháková K. 2015. Occurrence of the most common toxins vs. cytotoxicity in non-gasvacuolate heterocytous cianobacteria. Second EMBO Conference on Aquatic Microbial Ecology: SAME-14, Uppsala, Sweden

• Hrouzek P, Voráčová K, Hájek J, Mareš J, Urajová, P, Kuzma M, Cheel J, Villunger A, Kapuscik A, Bally M, Novák P, Kabeláč M, Krumschnabel G, Lukeš M, Voloshko L, Kopecký, J. 2015. A highly unusual organic scaffold, nocuolin A, produced by cyanobacteria triggers apoptosis and autophagy in human cancer cells. 15th International Symposium on Phototrophic Prokaryotes. Tübingen, Germany.

• Voráčová K, Villunger A, Kučerová P, Cheel J, Kapuscik A, Hrouzek P. 2014. Screening for novel bioactive metabolites in cyanobacteria: example of a novel apoptosis and autophagy inductor from cyanobacterium Nostoc sp. CALU 1533. Trends in Natural Products Research. Olomouc, Czech Republic.

• Hajek J, Urajova P, Hrouzek P. 2014. Production of diverse β-amino fatty acid containing lipopeptides by soil cyanobacteria of genus Cylindrospermum. III International Conference on Antimicrobial Research, Madrid, Spain.

• Voráčová K, Hrouzek P, Kučerová P, Kapuscik A, Cheel J, Kopecký J. 2013.Novel apoptosis inductor from Cyanobacterium Nostoc sp. Calu 1533. 4th BCNP Brazilian Conference on Natural Products. Natal-RN/Brasil.

• Hrouzek P, Kapuscik A, Voracova K, Tomek P, Kopecky J. 2011. Cyanobacterial cytotoxins as potential pharmaceutic agents and toxins. 8th European Workshop on Molecular Biology of Cyanobacteria, Naanthali, Finland.

Key current international cooperation

• Dr. David Fewer, Prof. Kaarina Sivonen (Department of Food and Environmental Sciences, University of Helsinki, Finsko)